Análisis Genómico Comparativo In Silico De Mycobacterium Tuberculosis

Torres Cabra, Yarith Alejandra; Hernández Mateus, Laura Natalia

Publicación:
Análisis Genómico Comparativo In Silico De Mycobacterium Tuberculosis

dc.contributor.advisor	Parra Muñoz Muñoz, Diana Marcela
dc.contributor.advisor	González Gómez, Andrés Camilo
dc.contributor.author	Torres Cabra, Yarith Alejandra
dc.contributor.author	Hernández Mateus, Laura Natalia
dc.date.accessioned	2026-05-07T23:37:06Z
dc.date.issued	2025-10
dc.description.abstract	La tuberculosis TB, es una enfermedad muy importante en el ambiente de la salud pública a nivel mundial, dado el impacto en las personas inmunosuprimidas, su fácil diseminación y el incremento de cepas resistentes, lo que permite evidenciar la gran capacidad adaptativa de Mycobacterium tuberculosis (MTB) (1). Este trabajo de grado presenta un análisis genómico comparativo in silico de 24 aislamientos clínicos colombianos frente a la cepa de referencia H37Rv, con el objetivo de identificar variantes genómicas asociadas con mecanismos de resistencia, virulencia y evasión inmune. Los resultados muestran la presencia de sustituciones y polimorfismos de un solo nucleótido (SNPs), que pueden causar eventos de extensión, truncación y pérdida del codón de inicio, mostrando una marcada diversidad genética entre los aislamientos, así como la presencia de dos linajes con características evolutivas diferentes. Estos hallazgos resaltan la importancia de investigar más a fondo el comportamiento molecular de MTB en Colombia, mediante herramientas de genómica comparativa que permite comprender los mecanismos que determinan la patogenicidad del patógeno.	spa
dc.description.degreelevel	Pregrado
dc.description.degreename	Bacteriólogo(a) y Laboratorista Clínico
dc.description.tableofcontents	Tabla de contenido RESUMEN 7 INTRODUCCIÓN 8 OBJETIVOS 11 Objetivo general 11 Objetivos específicos 11 1. ANTECEDENTES 12 2. MARCO REFERENCIAL 13 2.1. Filogenia de Mycobacterium tuberculosis 13 2.1.1. Características microbiológicas 14 2.1.2. Características genómicas 14 2.1.2.1 Epidemiologia molecular 15 2.1.2.1.1 Genotipos circulantes de Mycobacterium tuberculosis 21 2.2 Tuberculosis en Colombia 22 2.2.1 Situación epidemiológica 22 2.2.2 Métodos de diagnóstico molecular 24 2.2.3 Diagnóstico en poblaciones de alto riesgo 25 2.2.4 Tratamiento actual empleado 25 2.3 Mecanismos de resistencia 26 2.4 Mecanismos de virulencia 28 2.5 Genómica comparativa de Micobacterias 30 3. DISEÑO METODOLÓGICO 30 3.1 Búsqueda de genomas completos de Mycobacterium tuberculosis de aislamientos de pacientes colombianos. 31 3.2 Anotación estructural de regiones génicas de los genomas de MTB de aislamientos de pacientes colombianos. 32 3.3 Análisis de identidad de los genomas seleccionados de Mycobacterium tuberculosis 32 3.4 Análisis de genómica comparativa de aislamientos de Mycobacterium tuberculosis respecto a la cepa de referencia mundial H37Rv. 33 3.5 Análisis filogenético 33 4. RESULTADOS 34 4.1 Anotación estructural de regiones génicas de los genomas de Mycobacterium tuberculosis de aislamientos de pacientes colombianos 35 4.2 Análisis de identidad de los genomas seleccionados de Mycobacterium tuberculosis 38 4.3 Análisis de genómica comparativa de aislamientos de Mycobacterium tuberculosis respecto a la cepa de referencia mundial H37Rv. 39 4.4 Análisis filogenético 42 5. DISCUSIÓN 43 6. CONCLUSIONES 54 7. LIMITACIONES Y RECOMENDACIONES DEL ESTUDIO 54 8. BIBLIOGRAFÍA 55 9. ANEXOS 63	spa
dc.format.extent	63p.
dc.format.mimetype	application/pdf
dc.identifier.uri	https://repositorio.universidadmayor.edu.co/handle/unicolmayor/7387
dc.language.iso	spa
dc.publisher	Universidad Colegio Mayor de Cundinamarca
dc.publisher.faculty	Facultad de Ciencias de la Salud
dc.publisher.place	BOGOTÁ D.C
dc.publisher.program	Bacteriología y Laboratorio Clínico
dc.relation.references	World Health Organization: WHO. Tuberculosis [Internet]. Who.int. World Health Organization: WHO; 2019. Disponible en:: https://www.who.int/es/news-room/fact-sheets/detail/tuberculosis
dc.relation.references	World Health Organization. Global Tuberculosis Report 2024 [Internet]. Who.int. 2024. Disponible en:: https://www.who.int/teams/global-programme-on-tuberculosis-and-lunghealth/tb-reports/global-tuberculosis-report-2024
dc.relation.references	Coordinador H, Sepúlveda M, Subdirector C, Yaneth R, Acevedo, Ruge D. Informe de Evento 2024 Tuberculosis INSTITUTO NACIONAL DE SALUD Elaborado por: Revisado por: Aprobado por [Internet]. 2024. Disponible en:: https://www.ins.gov.co/buscadoreventos/Informesdeevento/TUBERCULOSIS%20INFORME%20DE%20EVENTO%202024.p df
dc.relation.references	Gygli SM, Borrell S, Trauner A, Gagneux S. Antimicrobial resistance in Mycobacterium tuberculosis: mechanistic and evolutionary perspectives. FEMS Microbiology Reviews [Internet]. 2017 Mar 25;41(3):354–73. Disponible en:: https://academic.oup.com/femsre/article/41/3/354/3089982
dc.relation.references	Chin KL, Anibarro L, Chang ZY, Praneetha Palasuberniam, Mustapha ZA, Sarmiento ME, et al. Impacts of MDR/XDR-TB on the global tuberculosis epidemic: Challenges and opportunities. Current Research in Microbial Sciences. 2024 Oct 1;7:100295–5.
dc.relation.references	Victoria MLM, Victoria MLM. Genes y determinantes de virulencia de Mycobacterium tuberculosis que contribuyen a la evasión de la respuesta inmune. Vaccimonitor [Internet]. 2020 [cited 2025 Nov 17];29(2):82–92. Disponible en:: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2020000200082
dc.relation.references	Zaw MT, Emran NA, Lin Z. Mutations inside rifampicin-resistance determining region of rpoB gene associated with rifampicin-resistance in Mycobacterium tuberculosis. Journal of infection and public health [Internet]. 2018;11(5):605–10. Disponible en:: https://www.ncbi.nlm.nih.gov/pubmed/29706316
dc.relation.references	Ioerger TR, Feng Y, Ganesula K, Chen X, Dobos KM, Fortune S, et al. Variation among Genome Sequences of H37Rv Strains of Mycobacterium tuberculosis from Multiple Laboratories. Journal of Bacteriology. 2010 Jul 15;192(14):3645–53.
dc.relation.references	JIANG Y, WEI J, LIU H, LI G, GUO Q, QIU Y, et al. Polymorphisms in the PE35 and PPE68 antigens in Mycobacterium tuberculosis strains may affect strain virulence and reflect ongoing immune evasion. Molecular Medicine Reports. 2015 Nov 19;13(1):947–54.
dc.relation.references	Bohada-Lizarazo DP, Bravo-Sanabria KD, Cárdenas-Malpica P, Raúl Rodríguez. Comparative Genomic Analysis of Mycobacterium tuberculosis Isolates Circulating in North Santander, Colombia. Tropical Medicine and Infectious Disease. 2024 Aug 28;9(9):197–7.
dc.relation.references	Nikolayevskyy V, Kranzer K, Niemann S, Drobniewski F. Whole genome sequencing of Mycobacterium tuberculosis for detection of recent transmission and tracing outbreaks: A systematic review. Tuberculosis. 2016 May;98:77–85.
dc.relation.references	Niemann S, Supply P. Diversity and Evolution of Mycobacterium tuberculosis: Moving to Whole-Genome-Based Approaches. Cold Spring Harbor Perspectives in Medicine. 2014 Sep 4;4(12):a021188–8.
dc.relation.references	Leong KWC, Gautam SS, Pradhan M, Singh YI, KC R, Rajbhandari SK, et al. Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations. Genomics. 2022 Mar;114(2):110278.
dc.relation.references	Dorronsoro I, Torroba L. Microbiología de la tuberculosis. Anales del Sistema Sanitario de Navarra [Internet]. 2007;30. Disponible en:: http://scielo.isciii.es/pdf/asisna/v30s2/original5.pdf
dc.relation.references	Lusayda SC. Bot Detection [Internet]. Udes.edu.co. 2025 [cited 2025 Nov 17]. Disponible en:: https://repositorio.udes.edu.co/server/api/core/bitstreams/564d855a-c19a-421d-8184- d59547a3032c/content
dc.relation.references	Technical Data. Please refer disclaimer Overleaf. M198 [Internet]. [cited 2025 Nov 17]. Disponible en:: https://www.himedialabs.com/media/TD/M198.pdf
dc.relation.references	Delogu G, Sali M, Fadda G. The Biology of Mycobacterium tuberculosis Infection. Mediterranean Journal of Hematology and Infectious Diseases [Internet]. 2013 Nov 15;5(1). Disponible en:: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867229/
dc.relation.references	Anaximandro Gómez‐Velasco, Molina-Torres CA, Vera-Cabrera L, Alied Bencomo-Alerm, Muñoz-Jiménez SG, Héctor Javier Sánchez-Pérez, et al. Diversidad genética del Complejo de Mycobacterium tuberculosis: implicaciones clínicas y epidemiológicas. Tip revista especializada en ciencias químico-biológicas. 2023 Jun 29;26.
dc.relation.references	Cubillos-Ruiz A, Juan Carlos Morales, Zambrano M. Analysis of the genetic variation in Mycobacterium tuberculosis strains by multiple genome alignments. BMC Research Notes. 2008 Nov 7;1(1):110–0.
dc.relation.references	Roychowdhury T, Mandal S, Bhattacharya A. Analysis of IS6110 insertion sites provide a glimpse into genome evolution of Mycobacterium tuberculosis. Scientific Reports. 2015 Jul 28;5(1).
dc.relation.references	Comín J, Otal I, Samper S. In-depth Analysis of IS6110 Genomic Variability in the Mycobacterium tuberculosis Complex. Frontiers in Microbiology. 2022 Feb 24;13.
dc.relation.references	Artículo De Revisión, Molecular B, Dilia Fontalvo Rivera, Doris Gómez Camargo. médicas uis revista de los estudiantes de medicina de la universidad industrial de santander Genes del Mycobacterium tuberculosis involucrados en la patogenicidad y resistencia a antibióticos durante la tuberculosis pulmonar y extrapulmonar [Internet]. 2015. Disponible en: http://www.scielo.org.co/pdf/muis/v28n1/v28n1a04.pdf
dc.relation.references	Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature [Internet]. 1998 Jun;393(6685):537–44. Disponible en: https://www.nature.com/articles/31159
dc.relation.references	Oudghiri A, Chaoui I, Elmzibri M. Molecular Epidemiology of Tuberculosis: A Review of Tools and Applications [Internet]. Omicsonline.org. 2025 [cited 2025 Nov 17]. Disponible en: https://www.omicsonline.org/open-access/molecular-epidemiology-of-tuberculosis-a-review-oftools-and-applications-2332-0877-1000386.pdf
dc.relation.references	Walker TM, Ip CL, Harrell RH, Evans JT, Kapatai G, Dedicoat MJ, et al. Whole-genome sequencing to delineate Mycobacterium tuberculosis outbreaks: a retrospective observational study. The Lancet Infectious Diseases [Internet]. 2013 Feb [cited 2019 Jul 29];13(2):137–46. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556524/
dc.relation.references	Coll F, McNerney R, José Afonso Guerra-Assunção, Glynn JR, João Perdigão, Viveiros M, et al. A robust SNP barcode for typing Mycobacterium tuberculosis complex strains. Nature Communications. 2014 Sep 1;5(1).
dc.relation.references	Coscolla M, Gagneux S. Consequences of genomic diversity in Mycobacterium tuberculosis. Seminars in Immunology. 2014 Dec;26(6):431–44.
dc.relation.references	Guyeux C, Senelle G, Meur AL, Supply P, Gaudin C, Phelan JE, et al. Early Release - Newly Identified Mycobacterium africanum Lineage 10, Central Africa - Volume 30, Number 3—March 2024 - Emerging Infectious Diseases journal - CDC. wwwnccdcgov [Internet]. 2024 May;30. Disponible en: https://wwwnc.cdc.gov/eid/article/30/3/23-1466_article
dc.relation.references	Rodríguez-Castillo JG, Llerena C, Argoty-Chamorro L, Guerra J, Couvin D, Rastogi N, et al. Population structure of multidrug-resistant Mycobacterium tuberculosis clinical isolates in Colombia. Tuberculosis. 2020 Dec;125:102011.
dc.relation.references	Hurtado-Páez U, Álvarez Zuluaga N, Arango Isaza RE, Contreras-Moreira B, Rouzaud F, Robledo J. Pan-genome association study of Mycobacterium tuberculosis lineage-4 revealed specific genes related to the high and low prevalence of the disease in patients from the NorthEastern area of Medellín, Colombia. Frontiers in microbiology [Internet]. 2023 Apr;13:1076797. Disponible en: https://pubmed.ncbi.nlm.nih.gov/36687645/
dc.relation.references	Cabezas Vinueza L, Jiménez Arias P. Distribution of Mycobacterium tuberculosis lineages in South America. Anatomía Digital. 2021 Jul 5;4(3):34–58.
dc.relation.references	Ferro B. Multidrug-Resistant Mycobacterium tuberculosis, Southwestern Colombia. Emerging Infectious Diseases. 2011 Jul;17(7):1259–62.
dc.relation.references	García DF, Astudillo M. Genotipificación de aislamientos del complejo Mycobacterium tuberculosis mediante MIRU-VNTR, Cali, Colombia, 2013-2015. Biomédica. 2019 May 1;39:71–85.
dc.relation.references	Puerto G, Erazo L, Wintaco M, Castro C, Ribón W, Guerrero MI. Mycobacterium tuberculosis Genotypes Determined by Spoligotyping to Be Circulating in Colombia between 1999 and 2012 and Their Possible Associations with Transmission and Susceptibility to First-Line Drugs. Cloeckaert A, editor. PLOS ONE. 2015 Jun 11;10(6):e0124308.
dc.relation.references	Pérez-Llanos FJ, Dreyer V, Barilar I, Utpatel C, Kohl TA, Murcia MI, et al. Transmission Dynamics of a Mycobacterium tuberculosis Complex Outbreak in an Indigenous Population in the Colombian Amazon Region. Microbiology Spectrum [Internet]. 2023 Jun 15;11(3):e0501322. Disponible en: https://pubmed.ncbi.nlm.nih.gov/37222610/
dc.relation.references	INSTITUTO NACIONAL DE SALUD. TUBERCULOSIS BOLETIN EPIDEMIOLÓGICO SEMANA 12 [Internet]. INSTITUTO NACIONAL DE SALUD. INSTITUTO NACIONAL DE SALUD; 2025. Disponible en: https://www.ins.gov.co/buscadoreventos/BoletinEpidemiologico/2025_Boletin_epidemiologico_semana_12.pdf
dc.relation.references	Amin M, Afrazeh E, Seyyedeh Fatemeh Seyyedian-Nikjeh, Meskini M, Delaram Doroud, Seyed Davar Siadat. New insight in molecular detection of Mycobacterium tuberculosis. AMB express. 2024 Jun 21;14(1).
dc.relation.references	Social P. REPUBLlCA DE COLOMBIA [Internet]. 2020. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/resolucion-227-de2020.pdf
dc.relation.references	Alejandra M, María A, Andrea C, Serrano G, Yaliana Tafur Cardona. Tuberculosis, Métodos Diagnósticos y su Validez. Revista Navarra Médica. 2018 Jan 5;3(2):15–23.
dc.relation.references	V Jarlier. Mycobacterial cell wall: Structure and role in natural resistance to antibiotics. FEMS Microbiology Letters. 1994 Oct 15;123(1-2):11–8.
dc.relation.references	Universidad de Buenos Aires. Bacterias que no toman la coloración de Gram -PARTE I Mycobacterium [Internet]. Disponible en: https://www.fmed.uba.ar/sites/default/files/2020- 07/T6A%20Texto%20Clase%206-Mycobacterium-Sordelli%202020.pdf
dc.relation.references	Heym B, Honoré N, Schurra C, Cole ST, Heym B, Truffot-Pernot C, et al. Implications of multidrug resistance for the future of short-course chemotherapy of tuberculosis: a molecular study. The Lancet. 1994 Jul;344(8918):293–8.
dc.relation.references	Gonzàlez-Martin J. Microbiología de la tuberculosis. Seminarios de la Fundación Española de Reumatología. 2014 Jan;15(1):25–33.
dc.relation.references	Poulton NC, Rock JM. Unraveling the mechanisms of intrinsic drug resistance in Mycobacterium 60 tuberculosis. Frontiers in Cellular and Infection Microbiology [Internet]. 2022 Oct 17 [cited 2023 May 8];12. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618640/
dc.relation.references	Nguyen L. Antibiotic resistance mechanisms in M. tuberculosis: an update. Archives of Toxicology. 2016 May 9;90(7):1585–604.
dc.relation.references	Ramón-Luing LA, Palacios Y, Ruiz A, Téllez-Navarrete NA, Chavez-Galan L. Virulence Factors of Mycobacterium tuberculosis as Modulators of Cell Death Mechanisms. Pathogens [Internet]. 2023 Jun 1;12(6):839. Disponible en: https://www.mdpi.com/2076-0817/12/6/839
dc.relation.references	Rahlwes Kathryn C, Dias Beatriz RS, Campos Priscila C, Alvarez-Arguedas S, Shiloh Michael U. Pathogenicity and Virulence of Mycobacterium tuberculosis. Virulence. 2022 Nov 23;14(1).
dc.relation.references	National Human Genome Research Institute. Comparative Genomics Fact Sheet [Internet]. Genome.gov. 2019 [cited 2025 Nov 17]. Disponible en: https://www.genome.gov/aboutgenomics/fact-sheets/Comparative-Genomics-Fact-Sheet?utm_source
dc.relation.references	Oppong A, Phelan J, João Perdigão, Machado D, Miranda A, Portugal I, et al. Genome-wide analysis of Mycobacterium tuberculosis polymorphisms reveals lineage-specific associations with drug resistance. BMC Genomics. 2019 Mar 29;20(1).
dc.relation.references	Gonzalo-Asensio J, Mostowy S, Harders-Westerveen J, Huygen K, Hernández-Pando R, Thole J, et al. PhoP: A Missing Piece in the Intricate Puzzle of Mycobacterium tuberculosis Virulence. Ahmed N, editor. PLoS ONE. 2008 Oct 23;3(10):e3496.
dc.relation.references	Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a Novel Method for Rapid Multiple Sequence Alignment Based on Fast Fourier Transform. Nucleic Acids Research. 2002 Jul 15;30(14):3059– 66.
dc.relation.references	Geneious. LASTZ - Geneious [Internet]. Geneious.com. 2020 [cited 2025 Nov 17]. Disponible en: https://www.geneious.com/plugins/lastz
dc.relation.references	Zhang Z, Dong L, Li X, Deng T, Wang Q. The PE/PPE family proteins of Mycobacterium tuberculosis: evolution, function, and prospects for tuberculosis control. Frontiers in Immunology. 2025 Jun 17;16.
dc.relation.references	Hall MB, Lima L, Coin LJM, Iqbal Z. Drug resistance prediction for Mycobacterium tuberculosis with reference graphs. Microb Genom [Internet]. 2023;9(8). Disponible en: https://www.microbiologyresearch.org/docserver/fulltext/mgen/9/8/mgen001081.pdf?expires=1 763685066&id=id&accname=guest&checksum=B8372AFF79B115A5E660BD665B1C9FDD
dc.relation.references	Luthra A, Gaur A, Ramachandran R. Rv3868 (EccA1), an essential component of the Mycobacterium tuberculosis ESX-1 secretion system, is thermostable. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics [Internet]. 2013 Feb 12;1834(6):1181–6. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S1570963913000678
dc.relation.references	Mikkel Illemann Johansen, Maiken Engelbrecht Petersen, Faddy E, Anders Marthinsen Seefeldt, Alexander Alexandrovich Mitkin, Lars Østergaard, et al. Efficacy of rifampicin combination therapy against MRSA prosthetic vascular graft infections in a rat model. Biofilm. 2024 Feb 1;100189–9.
dc.relation.references	Shukla S, Bhardwaj N, Singh A. Drug resistance in Mycobacterium tuberculosis: An evolutionary perspective and its adaptation to the lung microenvironment. The Microbe [Internet]. 2024 Oct 10;5:100189. Disponible en: https://www.sciencedirect.com/science/article/pii/S2950194624001560
dc.relation.references	Luthra A, Mahmood A, Arora A, Ramachandran R. Characterization of Rv3868, an essential hypothetical protein of the ESX-1 secretion system in Mycobacterium tuberculosis. The Journal of biological chemistry [Internet]. 2008;283(52):36532–41. Disponible en: https://pubmed.ncbi.nlm.nih.gov/18974091/
dc.relation.references	Domenech P, Reed MB, Barry CE. Contribution of the Mycobacterium tuberculosis MmpL Protein Family to Virulence and Drug Resistance. Infection and Immunity. 2005 Jun;73(6):3492– 501.
dc.relation.references	UNIPROT. UniProt [Internet]. UniProt. 2025 [cited 2025 Nov 17]. Disponible en: https://www.uniprot.org/uniprotkb/Q79FU2/entry
dc.relation.references	Chen T, Zhao Q, Li W, Xie J. Mycobacterium tuberculosis PE_PGRS17 Promotes the Death of Host Cell and Cytokines Secretion via Erk Kinase Accompanying with Enhanced Survival of Recombinant Mycobacterium smegmatis. Journal of Interferon & Cytokine Research. 2013 Aug;33(8):452–8.
dc.relation.references	Davies-Bolorunduro OF, Jaemsai B, Ruangchai W, Noppanamas T, Boonbangyang M, Bodharamik T, et al. Analysis of complete genomes of Mycobacterium tuberculosis sublineage 2.1 (Proto-Beijing) revealed the presence of three pe_pgrs3-pe_pgrs4-like genes. Scientific Reports [Internet]. 2024 Dec 28 [cited 2025 Jan 8];14(1). Disponible en: https://www.nature.com/articles/s41598-024-79351-w
dc.relation.references	Qian W, Ma N, Zeng X, Shi M, Wang M, Yang Z, et al. Identification of novel single nucleotide variants in the drug resistance mechanism of Mycobacterium tuberculosis isolates by whole- genome analysis. BMC Genomics. 2024 May 14;25(1).
dc.relation.references	De Maio F, Berisio R, Manganelli R, Delogu G. PE_PGRS proteins of Mycobacterium tuberculosis: A specialized molecular task force at the forefront of host–pathogen interaction. Virulence. 2020 Jan 1;11(1):898–915.
dc.relation.references	Suo J, Wang X, Zhao R, Ma P, Ge L, Luo T. Mycobacterium tuberculosis PPE7 Enhances Intracellular Survival of Mycobacterium smegmatis and Manipulates Host Cell Cytokine Secretion Through Nuclear Factor Kappa B and Mitogen-Activated Protein Kinase Signaling. Journal of Interferon & Cytokine Research. 2022 Oct 1;42(10):525–35.
dc.relation.references	Pérez E, Constant P, Lemassu A, Laval F, Daffé M, Guilhot C. Characterization of Three Glycosyltransferases Involved in the Biosynthesis of the Phenolic Glycolipid Antigens from the Mycobacterium tuberculosis Complex. Journal of Biological Chemistry. 2004 Oct;279(41):42574–83.
dc.relation.references	Ruaro-Moreno M, Monterrubio-López GP, Reyes-Gastellou A, Castelán-Vega JA, JiménezAlberto A, Aparicio-Ozores G, et al. Design of a Multi-Epitope Vaccine against Tuberculosis from Mycobacterium tuberculosis PE_PGRS49 and PE_PGRS56 Proteins by Reverse Vaccinology. Microorganisms [Internet]. 2023 Jul 1 [cited 2023 Dec 5];11(7):1647. Disponible en: https://www.mdpi.com/2076- 2607/11/7/1647#:~:text=Reverse%20vaccinology%20approaches%20use%20computational
dc.relation.references	Tiwari BM, Kannan N, Vemu L, Raghunand TR. The Mycobacterium tuberculosis PE Proteins Rv0285 and Rv1386 Modulate Innate Immunity and Mediate Bacillary Survival in Macrophages. Briken V, editor. PLoS ONE. 2012 Dec 17;7(12):e51686.
dc.relation.references	Katherine P, Yuk‐Ching Tse‐Dinh. Rv1495 toxin as a model for inhibitors of Mycobacterium tuberculosis DNA topoisomerase I. The FASEB Journal. 2018 Apr 1;32(S1).
dc.relation.references	Ahn DH, Lee KY, Sang Yup Lee, Sung Sup Park, Hye Eun Yoon, Kim SJ, et al. Structural analyses of the MazEF4 toxin-antitoxin pair in Mycobacterium tuberculosis provide evidence for a unique extracellular death factor. JOURNAL O F BIOLOGICAL CHEMISTRY. 2017 Nov 1;292(46):18832–47.
dc.relation.references	Quadri LEN. Biosynthesis of mycobacterial lipids by polyketide synthases and beyond. Critical Reviews in Biochemistry and Molecular Biology. 2014 Mar 14;49(3):179–211.
dc.relation.references	STRING. PKS4 [Internet]. STRING. Disponible en: https://string-db.org/network/83332.Rv1181
dc.relation.references	Tiwari B, Amarendranath Soory, Raghunand TR. An immunomodulatory role for the Mycobacterium tuberculosis region of difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873). FEBS Journal. 2014 Jan 28;281(6):1556–70.
dc.relation.references	D’Orazi G, Rinaldo C, Soddu S. Updates on HIPK2: a resourceful oncosuppressor for clearing cancer. BMC. 2012 Aug 13;31(1).
dc.relation.references	Qian J, Chen R, Wang H, Zhang X. Role of the PE/PPE Family in Host–Pathogen Interactions and Prospects for Anti-Tuberculosis Vaccine and Diagnostic Tool Design. Frontiers in Cellular and Infection Microbiology. 2020 Nov 26;10.
dc.relation.references	Leung KSS, Siu GKH, Tam KKG, To SWC, Rajwani R, Ho PL, et al. Comparative Genomic Analysis of Two Clonally Related Multidrug Resistant Mycobacterium tuberculosis by Single Molecule Real Time Sequencing. Frontiers in Cellular and Infection Microbiology. 2017 Nov 15;7.
dc.relation.references	Zhang J, Angala SK, Pramanik PK, Li K, Crick DC, Liav A, et al. Reconstitution of Functional Mycobacterial Arabinosyltransferase AftC Proteoliposome and Assessment of Decaprenylphosphorylarabinose Analogues as Arabinofuranosyl Donors. ACS Chemical Biology. 2011 May 26;6(8):819–28.
dc.relation.references	Hu Y, Ding Y, Cai B, Qin X, Wu J, Yuan M, et al. Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast. Cell Host & Microbe. 2022 Apr;30(4):518- 529.e6.
dc.relation.references	Mycobrowser [Internet]. Epfl.ch. 2019 [cited 2025 Nov 17]. Disponible en: https://mycobrowser.epfl.ch/genes/Rv0930
dc.rights.license	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.proposal	resistencia	spa
dc.subject.proposal	virulencia	spa
dc.subject.proposal	mutaciones	spa
dc.subject.proposal	SNPs	spa
dc.subject.proposal	filogenia	spa
dc.subject.proposal	variantes	spa
dc.title	Análisis Genómico Comparativo In Silico De Mycobacterium Tuberculosis	spa
dc.type	Trabajo de grado - Pregrado
dc.type.coar	http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content	Text
dc.type.driver	info:eu-repo/semantics/bachelorThesis
dc.type.redcol	http://purl.org/redcol/resource_type/TP
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication

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