Publicación: Epidemiología molecular de Mpox virus en Colombia
| dc.contributor.advisor | González, Andres Camilo | |
| dc.contributor.advisor | Parra Muñoz, Marcela | |
| dc.contributor.author | Pinto Garzón, Miguel Ángel | |
| dc.date.accessioned | 2026-05-06T16:39:30Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | El Mpox virus es un virus de ADN de doble cadena perteneciente a la familia Poxviridae del género Orthopoxvirus. Desde mayo del 2022 los casos de esta enfermedad aumentaron de manera significativa en países no endémicos siendo confirmados en la actualidad más de 100.000 casos en todo el mundo. En Colombia, los primeros casos se registraron en junio del mismo año, y desde entonces se han reportado un total de 4.288 casos en el territorio nacional. Con el fin de identificar los sublinajes del virus en el país, se determinó a través de un enfoque bioinformático la distribución y polimorfismos clave de 154 genomas de aislamientos de pacientes almacenados en el GISAID y NCBI entre el año 2022 y 2023. Asimismo, se evaluó el índice de adaptación de codones respecto al hospedero humano con el fin de reconocer estrategias adaptativas. Dentro de los resultados se hallaron 11 sublinajes los cuales se distribuyen en 12 regiones del país de manera heterogénea, observándose con predominancia los sublinajes B.1.10, B.1.11 y B.1.16 en la mayoría de estas. Por otra parte, se hallaron polimorfismos clave que afectan genes implícitos en la modulación de la respuesta inmune del huésped y su capacidad de adaptación. | spa |
| dc.description.degreelevel | Pregrado | |
| dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | |
| dc.description.tableofcontents | 1. Resumen 5 2. Introducción 5 3. Objetivos 7 3.1 Objetivo General 7 3.2 Objetivos Específicos 7 4. Justificación 8 5. Antecedentes 9 6. Marco teórico 11 6.1. Generalidades de la enfermedad y epidemiología 11 6.1.1. Características clínicas 11 6.1.2 Transmisión 13 6.1.3 Reservorio 14 6.1.4 Epidemiología e historia de la enfermedad 15 6.2 Características generales de Mpox virus 20 6.2.1 Patogénesis y proceso de replicación 21 6.2.2 Filogenia del virus 23 7. Metodología 25 7.1 Selección de secuencias 26 7.2 Análisis filogenético y distribución 27 7.3 Análisis de polimorfismos 27 7.4 Análisis de uso de codones 28 8. Resultados 28 8.1. Selección de secuencias 29 8.2 Análisis filogenético y distribución 29 8.3 Análisis de polimorfismos 33 8.4 Análisis de uso de codones 37 9. Discusión 38 10. Conclusión. 46 11. Referencias bibliográficas 47 12. Anexos 52 | spa |
| dc.format.extent | 53p. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://repositorio.universidadmayor.edu.co/handle/unicolmayor/7359 | |
| dc.language.iso | spa | |
| dc.publisher | Universidad Colegio Mayor de Cundinamarca | |
| dc.publisher.faculty | Facultad de Ciencias de la Salud | |
| dc.publisher.place | Bogotá D.C, | |
| dc.publisher.program | Bacteriología y Laboratorio Clínico | |
| dc.relation.references | Kmiec D, Kirchhoff F. Monkeypox: A New Threat? Vol. 23, International Journal of Molecular Sciences. MDPI; 2022. | |
| dc.relation.references | Marcela C, Lozada M, Alberto J, Reales M, Fuertes M, Jenessa Y, et al. Protocolo de Vigilancia de Viruela Símica-Monkeypox. 2022 | |
| dc.relation.references | Happi C, Adetifa I, Mbala P, Njouom R, Nakoune E, Happi A, et al. Urgent need for a non- discriminatory and non-stigmatizing nomenclature for monkeypox virus. PLoS Biol. 2022 Aug 1;20(8). | |
| dc.relation.references | Luna N, Ramírez AL, Muñoz M, Ballesteros N, Patiño LH, Castañeda SA, et al. Phylogenomic analysis of the monkeypox virus (MPXV) 2022 outbreak: Emergence of a novel viral lineage? Travel Med Infect Dis. 2022 Sep 1;49. | |
| dc.relation.references | Desingu PA, Rubeni TP, Sundaresan NR. Evolution of monkeypox virus from 2017 to 2022: In the light of point mutations. Front Microbiol. 2022 Dec 14;13. | |
| dc.relation.references | Yadav P, Devasurmutt Y, Tatu U. Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability. Viruses. 2023 Jan 1;15(1). | |
| dc.relation.references | Isidro J, Borges V, Pinto M, Sobral D, Santos JD, Nunes A, et al. Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus. Nature Medicine 2022 28:8 [Internet]. 2022 Jun 24 [cited 2023 Jan 13];28(8):1569–72. Available from: https://www.nature.com/articles/s41591-022-01907-y | |
| dc.relation.references | Zhan XY, Zha GF, He Y. Evolutionary dissection of monkeypox virus: Positive Darwinian selection drives the adaptation of virus–host interaction proteins. Front Cell Infect Microbiol. 2023 Jan 13;12. | |
| dc.relation.references | Yu X, Shi H, Cheng G. Mpox Virus: Its Molecular Evolution and Potential Impact on Viral Epidemiology. Vol. 15, Viruses. MDPI; 2023. | |
| dc.relation.references | Wang L, Shang J, Weng S, Aliyari SR, Ji C, Cheng G, et al. Genomic annotation and molecular evolution of monkeypox virus outbreak in 2022. J Med Virol. 2023 Jan 1;95(1). | |
| dc.relation.references | Guan H, Gul I, Xiao C, Ma S, Liang Y, Yu D, et al. Emergence, phylogeography, and adaptive evolution of mpox virus. New Microbes New Infect. 2023 Mar 1;52. | |
| dc.relation.references | Guo X, Zou J, Yang K, Chang S, Zhang Y, Li Y, et al. Non-adaptive evolution in codon usage of human-origin monkeypox virus. Comp Immunol Microbiol Infect Dis. 2023 Sep 1;100. | |
| dc.relation.references | Damon IK. Status of human monkeypox: Clinical disease, epidemiology and research. Vaccine. 2011 Dec 30;29(SUPPL. 4). | |
| dc.relation.references | Wilson ME, Hughes JM, McCollum AM, Damon IK. Human monkeypox. Clinical Infectious Diseases. 2014 Jan 15;58(2):260–7. | |
| dc.relation.references | Guzzetta G, Mammone A, Ferraro F, Caraglia A, Rapiti A, Marziano V, et al. Early Estimates of Monkeypox Incubation Period, Generation Time, and Reproduction Number, Italy, May-June 2022. Emerg Infect Dis. 2022 Oct 1;28(10):2078–81. | |
| dc.relation.references | McFarland SE, Marcus U, Hemmers L, Miura F, Iñigo Martínez J, Martínez FM, et al. Estimated incubation period distributions of mpox using cases from two international European festivals and outbreaks in a club in Berlin, May to June 2022. Euro Surveill. 2023 Jul 1;28(27). | |
| dc.relation.references | Huang Y, Mu L, Wang W. Monkeypox: epidemiology, pathogenesis, treatment and prevention. Vol. 7, Signal Transduction and Targeted Therapy. Springer Nature; 2022. | |
| dc.relation.references | Huhn GD, Bauer AM, Yorita K, Graham MB, Sejvar J, Likos A, et al. Clinical Characteristics of Human Monkeypox, and Risk Factors for Severe Disease. 2005. | |
| dc.relation.references | Walter K, Malani PN. What Is Monkeypox? Vol. 328, JAMA. American Medical Association; 2022. p. 222. | |
| dc.relation.references | Benites-Zapata VA, Ulloque-Badaracco JR, Alarcon-Braga EA, Hernandez-Bustamante EA, Mosquera-Rojas MD, Bonilla-Aldana DK, et al. Clinical features, hospitalisation and deaths associated with monkeypox: a systematic review and meta-analysis. Vol. 21, Annals of Clinical Microbiology and Antimicrobials. BioMed Central Ltd; 2022. | |
| dc.relation.references | Petersen E, Kantele A, Koopmans M, Asogun D, Yinka-Ogunleye A, Ihekweazu C, et al. Human Monkeypox: Epidemiologic and Clinical Characteristics, Diagnosis, and Prevention. Vol. 33, Infectious Disease Clinics of North America. W.B. Saunders; 2019. p. 1027–43. | |
| dc.relation.references | Guildo D, Eckburg P. Human monkeypox an emergin zoonosis. Lancet Infect Dis. 2004;4:15–25. | |
| dc.relation.references | Jezek Z, Szczeniowski M, Paluku KM, Mutombo M. Human Monkeypox: Clinical Features of 282 Patients [Internet]. Vol. 156, THE JOURNAL OF INFECTIOUS DISEASES. 1987. Available from: http://jid.oxfordjournals.org/ | |
| dc.relation.references | Rayati Damavandi A, Semnani F, Hassanpour K. A Review of Monkeypox Ocular Manifestations and Complications: Insights for the 2022 Outbreak. Vol. 12, Ophthalmology and Therapy. Adis; 2023. p. 55–69. | |
| dc.relation.references | Brown K, Leggat PA. Human monkeypox: Current state of knowledge and implications for the future. Vol. 1, Tropical Medicine and Infectious Disease. MDPI AG; 2016. | |
| dc.relation.references | Sklenovská N, Van Ranst M. Emergence of Monkeypox as the Most Important Orthopoxvirus Infection in Humans. Vol. 6, Frontiers in Public Health. Frontiers Media S.A.; 2018. | |
| dc.relation.references | Kumar N, Acharya A, Gendelman HE, Byrareddy SN. The 2022 outbreak and the pathobiology of the monkeypox virus. Vol. 131, Journal of Autoimmunity. Academic Press; 2022. | |
| dc.relation.references | Hutson CL, Carroll DS, Gallardo-Romero N, Weiss S, Clemmons C, Hughes CM, et al. Monkeypox disease transmission in an experimental setting: Prairie dog animal model. PLoS One. 2011 Dec 2;6(12). | |
| dc.relation.references | Kaler J, Hussain A, Flores G, Kheiri S, Desrosiers D. Monkeypox: A Comprehensive Review of Transmission, Pathogenesis, and Manifestation. Cureus. 2022 Jul 3; | |
| dc.relation.references | Heskin J, Belfield A, Milne C, Brown N, Walters Y, Scott C, et al. Transmission of monkeypox virus through sexual contact – A novel route of infection. Vol. 85, Journal of Infection. W.B. Saunders Ltd; 2022. p. 334–63. | |
| dc.relation.references | León-Figueroa DA, Barboza JJ, Garcia-Vasquez EA, Bonilla-Aldana DK, Diaz-Torres M, Saldaña-Cumpa HM, et al. Epidemiological Situation of Monkeypox Transmission by Possible Sexual Contact: A Systematic Review. Vol. 7, Tropical Medicine and Infectious Disease. MDPI; 2022. | |
| dc.relation.references | Pineda JER, Rojas SV, Ramírez JJO. Contagion of the monkeypox virus in men who have sex with men in Colombia: stigmatization. Salud(i)Ciencia. 2023;25(4):233–7. | |
| dc.relation.references | Fahrni ML, Priyanka, Choudhary OP. Possibility of vertical transmission of the human monkeypox virus. Vol. 105, International Journal of Surgery. Elsevier Ltd; 2022. | |
| dc.relation.references | Ullah M, Li Y, Munib K, Zhang Z. Epidemiology, host range, and associated risk factors of monkeypox: an emerging global public health threat. Vol. 14, Frontiers in Microbiology. Frontiers Media S.A.; 2023. | |
| dc.relation.references | Doty JB, Malekani JM, Kalemba LN, Stanley WT, Monroe BP, Nakazawa YU, et al. Assessing monkeypox virus prevalence in small mammals at the human–animal interface in the democratic republic of the congo. Viruses. 2017 Oct 1;9(10). | |
| dc.relation.references | Parker S, Nuara A, Buller RML, Schultz DA. Human monkeypox: An emerging zoonotic disease. Future Microbiol. 2007 Feb;2(1):17–34. | |
| dc.relation.references | Ladnyj D, Ziegler P, Kima3 E. A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo *. Vol. 46, Bull. Org. mond. Sante. 1972. | |
| dc.relation.references | Jeiek Z, Grab ’ B, Szczeniowski M, Paluku KM, Mutombo2 & M. Clinico-epidemiological features of monkeypox patients with an animal or human source of infection. Vol. 66, Bulltin of the World Health Organization. 1988. | |
| dc.relation.references | Heymann DL, Szczeniowski M, Esteves K. Re-emergence of monkeypox in Africa: a review of the past six years. Br Med Bull. 1998;54(3). | |
| dc.relation.references | Reynolds M, Davidson W, Curns A, Conover C, Huhn G, Davis J, et al. Spectrum of infection and risk factors for Human Monkeypox, United States, 2003. Emerg Infect Dis [Internet]. 2007;13(9). Available from: www.cdc.gov/eid | |
| dc.relation.references | Kabuga AI, El Zowalaty ME. A review of the monkeypox virus and a recent outbreak of skin rash disease in Nigeria. Vol. 91, Journal of Medical Virology. John Wiley and Sons Inc.; 2019. p. 533–40. | |
| dc.relation.references | Yinka-Ogunleye A, Aruna O, Ogoina D, Aworabhi N, Eteng W, Badaru S, et al. Reemergence of Human Monkeypox in Nigeria, 2017. Emerg Infect Dis. 2018;24(6). | |
| dc.relation.references | Yinka-Ogunleye A, Aruna O, Dalhat M, Ogoina D, McCollum A, Disu Y, et al. Outbreak of human monkeypox in Nigeria in 2017–18: a clinical and epidemiological report. Lancet Infect Dis. 2019 Aug 1;19(8):872–9. | |
| dc.relation.references | Hoff N, Doshi R, Colwell B, Kebela-Illunga B, Mukadi P, Mossoko M, et al. Evolution of a Disease Surveillance System: An Increase in Reporting of Human Monkeypox Disease in the Democratic Republic of the Congo, 2001-2013. Int J Trop Dis Health. 2017 Jan 10;25(2):1–10. | |
| dc.relation.references | Bunge EM, Hoet B, Chen L, Lienert F, Weidenthaler H, Baer LR, et al. The changing epidemiology of human monkeypox—A potential threat? A systematic review. PLoS Negl Trop Dis. 2022 Feb 1;16(2). | |
| dc.relation.references | Vaughan A, Aarons E, Astbury J, Balasegaram S, Beadsworth M, Beck C, et al. Two cases of monkeypox imported to the United Kingdom, September 2018. Eurosurveillance. 2018 Sep 21;23(38). | |
| dc.relation.references | WHO. Multi-country monkeypox outbreak in non-endemic countries [Internet]. 2022 [cited 2023 Jul 25]. Available from: https://www.who.int/emergencies/disease-outbreaknews/item/2022-DON385 | |
| dc.relation.references | Nuzzo JB, Borio LL, Gostin LO. The WHO Declaration of Monkeypox as a Global Public Health Emergency. Vol. 328, JAMA. American Medical Association; 2022. p. 615–6. | |
| dc.relation.references | CDC. Datos y casos del brote del 2022 [Internet]. 2023 [cited 2023 Jul 25]. Available from: https://espanol.cdc.gov/poxvirus/mpox/response/2022/index.html | |
| dc.relation.references | Otieno JR, Ruis C, Onoja AB, Kuppalli K, Hoxha A, Nitsche A, et al. Global genomic surveillance of monkeypox virus. Nat Med [Internet]. 2025 Jan 23;31(1):342–50. Available from: https://www.nature.com/articles/s41591-024-03370-3 | |
| dc.relation.references | CDC. Mpox [Internet]. 2024 [cited 2024 Nov 30]. Available from: https://www.cdc.gov/mpox/index.html | |
| dc.relation.references | INS. Enfermedades emergentes. 2024 [cited 2024 Nov 30]. Mpox. Available from: https://www.ins.gov.co/Noticias/Paginas/Enfermedades-emergentes.aspx | |
| dc.relation.references | Kugelman JR, Johnston SC, Mulembakani PM, Kisalu N, Lee MS, Koroleva G, et al. Genomic variability of monkeypox virus among humans, Democratic Republic of the Congo. Emerg Infect Dis. 2014 Feb;20(2):232–9. | |
| dc.relation.references | 51 Shchelkunov SN, Totmenin A V, Babkin I V, Safronov PF, Ryazankina OI, Petrov NA, et al. Human monkeypox and smallpox viruses: genomic comparison. 2001. | |
| dc.relation.references | Li H, Huang QZ, Zhang H, Liu ZX, Chen XH, Ye LL, et al. The land-scape of immune response to monkeypox virus [Internet]. 2022. Available from: www.thelancet.com | |
| dc.relation.references | Shchelkunov SN, Totmenin A V., Safronov PF, Mikheev M V., Gutorov V V., Ryazankina OI, et al. Analysis of the monkeypox virus genome. Virology. 2002;297(2):172–94. | |
| dc.relation.references | Krijnse J, Kuehn A, Schleich S, Rutter G, Hohenberg H, Wepf R, et al. Entry of the Two Infectious Forms of Vaccinia Virus at the Plasma Membane Is Signaling-Dependent for the IMV but Not the EEV. Vol. 11, Molecular Biology of the Cell. 2000. | |
| dc.relation.references | Bayer-Garner IB. Monkeypox virus: histologic, immunohistochemical and electronmicroscopic findings. | |
| dc.relation.references | Moss B. Membrane fusion during poxvirus entry. 2016; | |
| dc.relation.references | Hatmal MM, Al-Hatamleh MAI, Olaimat AN, Ahmad S, Hasan H, Ahmad Suhaimi NA, et al. Comprehensive literature review of monkeypox. Vol. 11, Emerging Microbes and Infections. Taylor and Francis Ltd.; 2022. p. 2600–31. | |
| dc.relation.references | Moss B. Poxvirus DNA replication. Cold Spring Harb Perspect Biol. 2013 Sep;5(9). | |
| dc.relation.references | Smith GL, Vanderplasschen A, Law M. Printed in Great Britain The formation and function of extracellular enveloped vaccinia virus [Internet]. Vol. 83, Journal of General Virology. 2002. Available from: www.sanger.ac.uk | |
| dc.relation.references | Li H, Zhang H, Ding K, Wang XH, Sun GY, Liu ZX, et al. The evolving epidemiology of monkeypox virus. Vol. 68, Cytokine and Growth Factor Reviews. Elsevier Ltd; 2022. p. 112. | |
| dc.relation.references | Turakhia Y, Thornlow B, Hinrichs AS, De Maio N, Gozashti L, Lanfear R, et al. Ultrafast Sample placement on Existing tRees (UShER) enables real-time phylogenetics for the SARS-CoV-2 pandemic. Nat Genet. 2021 Jun 1;53(6):809–16. | |
| dc.relation.references | Xie J, Chen Y, Cai G, Cai R, Hu Z, Wang H. Tree Visualization by One Table (tvBOT): A web application for visualizing, modifying and annotating phylogenetic trees. Nucleic Acids Res. 2023 Jul 5;51(W1):W587–92. | |
| dc.relation.references | Aksamentov I, Roemer C, Hodcroft E, Neher R. Nextclade: clade assignment, mutation calling and quality control for viral genomes. J Open Source Softw. 2021 Nov 30;6(67):3773. | |
| dc.relation.references | Puigbò P, Bravo IG, Garcia-Vallve S. CAIcal: A combined set of tools to assess codon usage adaptation. Biol Direct. 2008 Sep 16;3. | |
| dc.relation.references | Luna N, Muñoz M, Bonilla-Aldana DK, Patiño LH, Kasminskaya Y, Paniz-Mondolfi A, et al. Monkeypox virus (MPXV) genomics: A mutational and phylogenomic analyses of B.1 lineages. Travel Med Infect Dis. 2023 Mar 1;52. | |
| dc.relation.references | Patiño LH, Guerra S, Muñoz M, Luna N, Farrugia K, van de Guchte A, et al. Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022. Emerg Microbes Infect. 2023;12(1). | |
| dc.relation.references | Zhang S, Wang F, Peng Y, Gong X, Fan G, Lin Y, et al. Evolutionary trajectory and characteristics of Mpox virus in 2023 based on a large-scale genomic surveillance in Shenzhen, China. Nature Communications . 2024 Dec 1;15(1). | |
| dc.relation.references | Yu Z, Zou X, Deng Z, Zhao M, Gu C, Fu L, et al. Genome analysis of the mpox (formerly monkeypox) virus and characterization of core/variable regions. Genomics. 2024 Jan 1;116(1). | |
| dc.relation.references | Young B, Seifert SN, Lawson C, Koehler H. Exploring the genomic basis of Mpox virushost transmission and pathogenesis. Imperiale MJ, editor. mSphere [Internet]. 2024 Dec 19;9(12). Available from: https://journals.asm.org/doi/10.1128/msphere.00576-24 | |
| dc.relation.references | Yu J, Zhang X, Liu J, Xiang L, Huang S, Xie X, et al. Phylogeny and molecular evolution of the first local monkeypox virus cluster in Guangdong Province, China. Nat Commun. 2023 Dec 1;14(1). | |
| dc.rights | Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores. | spa |
| dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.subject.proposal | Mpox | spa |
| dc.subject.proposal | Polimorfismos | spa |
| dc.subject.proposal | Epidemiología molecular, | spa |
| dc.title | Epidemiología molecular de Mpox virus en Colombia | spa |
| dc.type | Trabajo de grado - Pregrado | |
| dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | |
| dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| dc.type.content | Text | |
| dc.type.driver | info:eu-repo/semantics/bachelorThesis | |
| dc.type.redcol | http://purl.org/redcol/resource_type/TP | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication |