Producción de astaxantina a partir de Haematococcus pluvialis en medio KM y  BBM usando luz led roja (650 nm) y led azul (450 nm)

Torres Martinez, Natalia Lorena; Cuellar Bogota, Mayra Tatiana

Publicación:
Producción de astaxantina a partir de Haematococcus pluvialis en medio KM y BBM usando luz led roja (650 nm) y led azul (450 nm)

dc.contributor.advisor	Camacho Kurmen, Judith Elena
dc.contributor.author	Torres Martinez, Natalia Lorena
dc.contributor.author	Cuellar Bogota, Mayra Tatiana
dc.date.accessioned	2026-05-06T15:43:30Z
dc.date.issued	2025
dc.description.abstract	La microalga Haematococcus pluvialis, es considerada como la fuente natural de astaxantina, al ser sometida a condiciones de estrés. La astaxantina, es un producto natural que tiene gran interés por ser utilizada como pigmento en alimentos, fuente de vitaminas, colorante, conservante natural, aditivo alimentario, antioxidante, antienvejecimiento, agente anticancerígeno y un inmunomodulador. El presente trabajo, tiene como objetivo determinar la producción de astaxantina en H. pluvialis usando medios de cultivo KM y BBM. En la etapa verde se usan condiciones favorables con una irradiancia de 130.65 μmol/m2 /s (1700 luxes) con luz led roja (650 nm). En la etapa roja se aplicó estrés con luz led azul (450 nm) con una irradiancia de 924.42 μmol/m2 /s (8000 luxes). Se realizó el ANOVA (95%) para establecer diferencias significativas entre tratamientos. Se encontró que el medio de cultivo BBM bajo las condiciones trabajadas demostró ser mejor en el crecimiento de la microalga con 2,88x106 cel./mL con velocidad de crecimiento (R2=0,87, modelo logístico) usando luz led roja (650 nm) por 25 días y luego luz led azul (450 nm) por 47 días observándose no hay diferencias significativas (F:2.051; P:0.174; gl:1). El tratamiento realizado en el medio KM produjo la mayor concentración de astaxantina 20,29 μg/mL, presentando quistes inmaduros y maduros de tonalidad rojiza por acumulación de este pigmento, usando luz led roja. observándose diferencias significativas (F:9.060; P:0.009; gl:1). Se estableció que el uso de la luz led roja y luz led azul incrementaron el crecimiento de la microalga y la acumulación de astaxantina.	spa
dc.description.degreelevel	Pregrado
dc.description.degreename	Bacteriólogo(a) y Laboratorista Clínico
dc.description.tableofcontents	Tabla de contenido Resumen 8 1. Introducción 9 2. Objetivos 10 2.1 Objetivo general 10 2.2 Objetivos específicos 10 3. Estado del arte 11 4. Marco teórico 20 4.1 Factores que afectan el crecimiento 22 4.2 Nutrientes 24 4.3 Factores de estrés 25 5. Astaxantina 26 5.1 Ruta metabólica de producción de astaxantina 26 5.2 Usos y aplicaciones 27 6. Hipótesis 27 7. Diseño metodológico 27 7.1 Fase 1. Crecimiento de la microalga H. pluvialis 28 7.2 Fase 2. Cambio morfológico 30 7.3 Fase 3. Determinación de clorofila y astaxantina 30 8. Resultados 31 8.1 Fase 1. Crecimiento de la microalga H. pluvialis 31 8.2 Parámetros cinéticos del crecimiento de H. pluvialis 32 8.3 Cambio morfológico 34 8.4 Producción de astaxantina y clorofila 36 8.5 Relación entre crecimiento y producción de pigmentos 37 8.6 pH 37 9. Discusión 39 10. Conclusiones 42 11. Recomendaciones 43 12. Participación en eventos 43 Bibliografía 44
dc.format.extent	57p.
dc.format.mimetype	application/pdf
dc.identifier.uri	https://repositorio.universidadmayor.edu.co/handle/unicolmayor/7358
dc.language.iso	spa
dc.publisher	Universidad Colegio Mayor de Cundinamarca
dc.publisher.faculty	Facultad de Ciencias de la Salud
dc.publisher.place	Bogota D.C
dc.publisher.program	Bacteriología y Laboratorio Clínico
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dc.rights	Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores	spa
dc.rights.license	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.proposal	Microalga	spa
dc.subject.proposal	Luz Led	spa
dc.subject.proposal	Astaxantina	spa
dc.subject.proposal	Condiciones de Estrés	spa
dc.title	Producción de astaxantina a partir de Haematococcus pluvialis en medio KM y BBM usando luz led roja (650 nm) y led azul (450 nm)
dc.type	Trabajo de grado - Pregrado
dc.type.coar	http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content	Text
dc.type.driver	info:eu-repo/semantics/bachelorThesis
dc.type.redcol	http://purl.org/redcol/resource_type/TP
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication

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